Dr. OWOLADE, O.F.
Head of Unit
CURRENT RESEARCH ACTIVITIES
- Development of High Yielding Quality Protein Maize (Zea Mays) Hybrids for Adaptation to Forest and Savanna Agro-Ecologies, Using Marker Assisted Techniques
- Genetic Improvement of Kenaf (Hibiscus cannabinus ) Seed via Accelerated Aging and Molecular Assisted Technique
- Genetic mapping of Callosobruchus maculatus (Coleoptera: Bruchidae) resistant gene in cowpea.
- Improving the levels of resistance to maize streak virus (MSV) in elite maize lines.
- Improvement of Kenaf (Hibiscus cannabinus ) Seed through Molecular Assisted Technique
- Characterization Swine leukocyte Antigen of Nigerian pigs
The 2018 in-house report of Biotechnology Unit
The Biotechnology Unit was established in the Institute to shortening the time needed for varietal development through conventional breeding techniques. There are three main aspects of biotechnology which have relevance for crop and animal improvement: The sub-themes of the unit are crop improvement through tissue culture techniques as well as plant and animal breeding through marker assisted selection. Cell and Tissue Culture is presently being used for micro propagation of planting materials as we have started with Kenaf/fibre in the Institute, disease elimination, microspore culture, conservation and in vitro selection, DNA markers are also being used for germplasm characterization and evolutionary study, marker assisted selection and QTL identification.
- In a bid to develop simple and cheap protocol for in vitro screening of low soil nitrogen tolerant maize varieties. One of the varieties TZPB PROI C3 was consistently more productive in the field and in vitro under low and high nitrogen applications. This indicates that maize line TZPB Prol C6 possesses favourable genes for low N-tolerance and it could be a good line for further low N tolerant in breeding programs. Therefore, this simple protocol for screening low Nitrogen tolerant maize through tissue culture technique can be adopted to compliment conventional maize breeding designed for low nitrogen environments.
- Tissue culture has successfully used to screen Quality Protein Maize against drought tolerance in vitro within a short time by using PEG-6000 as osmoticum when compared with field (in-vivo) screening that is time consuming and labour intensive. It can be inferred from this experiment that all the maize varieties Oba 98 and ILE-1-OB used in the study overcame the effect of imposed stress by PEG, therefore, they can be regarded as drought tolerant maize varieties.
- Cytogenetic characterization of Nigerian indigenous pig has been investigated. Information obtained will be of tremendous benefits in pig breeding programme.
- Genetic linkage map of cowpea (Vigna unguiculata (L.) Walp) using SNP markers among mapping population between IT-95K-193-12 and Ife-Brown was investigated. Results obtained revealed 12 linkage maps with an average of 10 markers to a chromosome and average marker distance of 3.02cM between the markers. Constructed map provides basic information that could assist in genetic improvement of cowpea, most especially in developing brown blotch resistant cowpea varieties through detection of quantitative trait loci (QTL) responsible for brown blotch resistance in cowpea when utilizing marker assisted selection method.
- Yellow maize lines with pro-vitamin A were characterized, and classified using SSR markers among some maize varieties developed at IITA and IAR&T. The study identified PVA8 and its most three distant relatives as potential divergent parents that could serve as important genetic resources for broadening the genetic base of the presently assessed maize collections and also to develop new maize lines with higher level of pro-vitamin A content.
- Effect of seed size on in vitro seed germination, seedling growth, embryogenic callus induction and plantlet regeneration from embryo of maize (Zea mays) seed has been investigated. Findings from this study are good information that can assist plant breeders to improve maize using biotechnology for efficient and rapid incorporation of relevant seed traits on desirable maize genotypes.
- Restriction Fragment Length Polymorphism (RFLP) and microsatellite DNA markers were used to establish that Colletotrichum gloesporioides is the causal agent of cassava anthacnose disease typified by stem cankers.
- Comparative assessment of quality of genomic DNA extracted from maize seed and leaf tissues has been investigated. DNA extracted from maize without the use of liquid nitrogen can be effectively used without any adverse effect on the quality of the result.
- Genetic purity analysis of Quality protein maize using molecular Techniques has been conducted and relationships among the QPM lines were evaluated.
- Physiological age of explants, basal salt composition and genotype were found to affect callus formation and plantlet regeneration as means of protocol optimization for indirect somatic embryogenesis.
- Comparison of African Swine Fever Virus prevalence in Nigerian Indigenous Pig, Its Hybrid and Backcross In an Environment prone to African Swine Fever. Result showed that ASFV was present in NIP, hybrid and the backcross using PCR primers PAS. The inflammation signal (white blood count, red blood count and platelet count) in hybrid was higher than that of NIP and backcross.
- Optimization of in vitro culture for shoot induction and multiplication from shoot tip and meristem culture of T. Occidentalis (Ugu) was accomplished. This is useful for developing uniform clone and production of virus free genotypes of the crops.